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Objective To analyze MRI features of intraspinal ganglioneuroma,in order to improve the ability of preoperative diagnosis.Methods MRI data of 9 patients of intraspinal ganglioneuroma confirmed pathologically were analyzed retrospectively.The location,morphology,size,signal intensity and degree of enhancement of these lesions were observed.Results Totally 9 lesions were enrolled.Four lesions occurred in the cervical spine,2 in the thoracic spine and 3 in the lumbosacral spine.Seven lesions were dumbbell shaped,involving both intra and extra-canalicular,and 2 lesions present as nodules in the foramen.All the lesions demonstrated homogeneous or heterogeneous high signal on T2WI,with only 1 lesion showed cystic change and necrosis.Contrast-enhanced scanning was performed in 8 lesions,and marked enhancement was shown in 3 lesions,mild-moderate enhancement in 5 lesions.Strand-shaped enhancement took place in 4 lesions.The intervertebral foramina of all the lesions were enlarged,but without bone destruction.Conclusion Intraspinal ganglioneuroma has some characteristics on MRI,which may be helpful to accurate preoperative diagnosis.
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Objective To investigate the clinicopathologic characteristics of and HPV subtypes in vulvar condyloma acuminatum(CA)and subclinical HPV infection.Methods Eighty patients with a positive acetowhite test and suspected subclinical HPV infection were selected from 272 patients with typical CA lesions in perianal and external genital region.Tissue specimens were obtained from typical CA and suspected subclinical HPV-infected lesions followed by pathological examination and HPV-DNA detection.Finally,71 patients were confirmed to suffer from both CA and subclinical HPV infection.A comparative analysis was performed to assess the differences in histopathological manifestation and HPV genotypes between CA and subclinical infection lesions.Results Pathological examination revealed typical histological changes of CA in 71(88.75％)typical CA specimens and 4(5％)suspected subclinical infection specimens,as well as squamous dysplasia in 9(11.25％)CA specimens and 71(88.75％)suspected subclinical infection specimens.HPV-DNA was positive in all(80)of the CA specimens and 93.75％(75)of the suspected subclinical infection specimens,negative in 5(6.25％)suspected subclinical infection specimens.Obvious differences were observed in pathological manifestation,koilocyte number(P ＜ 0.05),but not in the distribution of HPV subtypes(P ＞ 0.05),between typical CA and concurrent subclinical HPV infection lesions in patients.Conclusion The diagnosis of subclinical HPV infection should be based on the result of acetowhite test,with the results of pathological examination and HPV DNA detection as an adjuvant.
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BACKGROUND:Basic fibroblast growth factor(bFGF)can promote production of collagen,fibronectin and matrix enzyme in healing wounds.However,dysregulation of this process,such as the abnormal coordination of cell proliferation,extracellular.matrix and neovasculadzation formation,or remodeling of the wound matrix will lead to excess accumulation of scar tissues.OBJECTIVE:To investigate effects of bFGF on normal skin wound healing and hypertrophic scar formation.METHODS:Normal and hypertrophic scar fibroblasts from tissue biopsies from 5 patients who underwent plastic surgery for repairing hypertrophic scars were isolated and cultured.The expressions of collagen,fibronectin and protein synthesis were detected by RT-PCR and ELISA.The mitochonddal membrane potential changes were measured using JC-1 staining and flow cytometry.Simultaneously,adenosine tdphosphate(ATP)levels were determined by chemiluminescence method.The effects of bFGF on these indexes of normal and hypertrophic scar fibroblasts were observed.RESULTS AND CONCLUSION:Hypertrophic scar fibroblasts become slower after being exposed to bFGF,which selectively inhibited type Ⅰ collagen production in hypertrophic scar fibroblasts(P<0.05).Although bFGF inhibited type]collagen production,it had no effect on type Ⅲ collagen expression in both normal and hypertrophic scar fibroblasts.However,fibronectin expression in the normal fibroblasts was up-reguleted after bFGF treatment(P<0.05).In addition,the mitochonddal membrane potential tended to depolarization,although no statistical difference,in hypertrophic scar fibroblasts treated with bFGF(10 or 100 μg/L).bFGF treatment increased the cellular ATP levels in the normal fibroblasts,while there were no significant alterations in the hypertrophic scar fibroblasts over a treatment of bFGF(10 or 100 μg/L,P<0.05).The results suggest that there are differential effects and mechanisms on the skin fibroblasts with bFGF treatment in normal wound healing and hypertrophic scar formation.
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[Objective]This study was designed to discover the expression of histamine H4 receptor in allergic rhinitis(AR)and its clinical significance.[Methods]Seventy-three patients with allergic rhinitis and 30 volunteers were recruited in our study.Western blot and Real-time fluorescent quantification PCR were used to detect the protein and mRNA expression of H4R on peripheral blood monocytes(PBMC).To analyze the relationship between the H4R and allergic rhinitis,the level of serum interleukin 4(IL-4)and interleukin 12(IL-12)were determined by enzyme linked immunosorbnent assay(ELISA)in the AR group.[Results]The protein and mRNA expression of H4R in the AR group were significantly higher than that in the NAR group.Person correlation analysis revealed that H4R was positively correlated with IL-4(r=0.71,P＜0.01)and negatively correlated with IL-12(r=-0.50,P＜0.01).The expression of H4R also had positive relationship with IL-4/IL-12 ratio(r=0.75,P＜0.01).[Conclusion]These results indicate that histamine H4 receptor is closely related with allergic rhinitis and is important in the pathogenesis of AR.The elevated expression of H4R in AR patients' PBMC may be closely correlated with Th1/Th2 balance.
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Objective To assess the clinical value of 16-slice spiral CT in the diagnosis of multiple carcinomas in unilateral upper urinary system. Methods The imaging findings of 16-slice spiral CT of 10 patients suffered from multiple carcinomas in the unilateral upper urinary system proven pathologically were analyzed. Results Among 10 cases, 6 cases had multiple carcinomas in the unilateral renal pelvis and ureter and simultaneously involved urinary bladder, 3 cases had the multiple carcinomas in the unilateral renal pelvis and ureter, and 1 case had the multiple carcinomas in the lower and middle ureter and involved urinary bladder. CT images clearly presented the thickened irregular renipelvic and ureteric wall with stenosis and/or mass in the renipelvic and ureteric cavity. The urinary bladders involved by carcinoma were depicted as thickened irregular wall of urinary bladder or the mass in the wall of urinary bladder. The images of curved reconstruction clearly showed the extention of the multiple carcinomas and the changes of the urinary tract involved by the multiple carcinomas. Enlarged retroabdominal lymph nodes were detected in 3 cases simultaneously, and 2 of them were proved metastasis, the imaging findings were absolutely identical to that of the operation and pathological examination. Conclusion Sixteen slice spiral CT can clearly and accurately depict the location and extention of multiple carcinomas in the unilateral upper urinary system.